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rhil21  (Pfizer Inc)

 
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    Structured Review

    Pfizer Inc rhil21
    Whole human blood response to ex vivo stimulation with 10 ng/mL <t>rhIL21</t> for 2 hours . Data shown are for the 6 genes (of 19 tested) with the most consistent response among the 9 individual donors, and are identified as 6 preferred biomarkers of rhIL21 activity in whole human blood. These 6 genes were also identified as the best indicators of IL21 response in a series of pilot studies conducted with blood from a different group of donors (data not shown).
    Rhil21, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rhil21/product/Pfizer Inc
    Average 90 stars, based on 1 article reviews
    rhil21 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood"

    Article Title: Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

    Journal: Journal of Translational Medicine

    doi: 10.1186/1479-5876-8-51

    Whole human blood response to ex vivo stimulation with 10 ng/mL rhIL21 for 2 hours . Data shown are for the 6 genes (of 19 tested) with the most consistent response among the 9 individual donors, and are identified as 6 preferred biomarkers of rhIL21 activity in whole human blood. These 6 genes were also identified as the best indicators of IL21 response in a series of pilot studies conducted with blood from a different group of donors (data not shown).
    Figure Legend Snippet: Whole human blood response to ex vivo stimulation with 10 ng/mL rhIL21 for 2 hours . Data shown are for the 6 genes (of 19 tested) with the most consistent response among the 9 individual donors, and are identified as 6 preferred biomarkers of rhIL21 activity in whole human blood. These 6 genes were also identified as the best indicators of IL21 response in a series of pilot studies conducted with blood from a different group of donors (data not shown).

    Techniques Used: Ex Vivo, Activity Assay

    Determination of optimal time point for rhIL21-response for the 6 genes identified as preferred biomarkers . This time point experiment was done using 5 of the donors shown in Figure 2. Earlier pilot studies had suggested that stimulation for 30 minutes was significantly sub-optimal. There was no significant difference in response between all doses tested (30, 10, and 3 ng/mL). The lowest dose of rhIL21 that elicits a response has therefore not been determined.
    Figure Legend Snippet: Determination of optimal time point for rhIL21-response for the 6 genes identified as preferred biomarkers . This time point experiment was done using 5 of the donors shown in Figure 2. Earlier pilot studies had suggested that stimulation for 30 minutes was significantly sub-optimal. There was no significant difference in response between all doses tested (30, 10, and 3 ng/mL). The lowest dose of rhIL21 that elicits a response has therefore not been determined.

    Techniques Used:

    Average percent inhibition of the expression level of 6 IL21-responsive genes . Percent inhibition values were calculated based on RQ (relative quantification) values of untreated control and rhIL21-treated samples for each of the 4 donors, and subsequently the mean and standard deviation were determined for each gene shown. A: Percent inhibition in presence of Ab-01. B: Percent inhibition in presence of control IgG 1 TM. Data for the 0.1 μg/mL , 0.3 μg/mL and 1 μg/mL concentrations were generated using 4 donors. Data for the higher and the lower concentrations were generated using 2 donors.
    Figure Legend Snippet: Average percent inhibition of the expression level of 6 IL21-responsive genes . Percent inhibition values were calculated based on RQ (relative quantification) values of untreated control and rhIL21-treated samples for each of the 4 donors, and subsequently the mean and standard deviation were determined for each gene shown. A: Percent inhibition in presence of Ab-01. B: Percent inhibition in presence of control IgG 1 TM. Data for the 0.1 μg/mL , 0.3 μg/mL and 1 μg/mL concentrations were generated using 4 donors. Data for the higher and the lower concentrations were generated using 2 donors.

    Techniques Used: Inhibition, Expressing, Quantitative Proteomics, Control, Standard Deviation, Generated

    rhIL21-responsive genes in whole cynomolgus monkey blood
    Figure Legend Snippet: rhIL21-responsive genes in whole cynomolgus monkey blood

    Techniques Used:

    Inhibition by Ab-01 of ex vivo rhIL21-dependent IL2RA expression
    Figure Legend Snippet: Inhibition by Ab-01 of ex vivo rhIL21-dependent IL2RA expression

    Techniques Used: Inhibition, Ex Vivo, Control

    rhIL21 induced IL2RA expression in whole blood from cynomolgus monkeys dosed with Ab-01
    Figure Legend Snippet: rhIL21 induced IL2RA expression in whole blood from cynomolgus monkeys dosed with Ab-01

    Techniques Used: Expressing, Control



    Similar Products

    rhil21  (Pfizer Inc)
    90
    Pfizer Inc rhil21
    Whole human blood response to ex vivo stimulation with 10 ng/mL <t>rhIL21</t> for 2 hours . Data shown are for the 6 genes (of 19 tested) with the most consistent response among the 9 individual donors, and are identified as 6 preferred biomarkers of rhIL21 activity in whole human blood. These 6 genes were also identified as the best indicators of IL21 response in a series of pilot studies conducted with blood from a different group of donors (data not shown).
    Rhil21, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rhil21/product/Pfizer Inc
    Average 90 stars, based on 1 article reviews
    rhil21 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Whole human blood response to ex vivo stimulation with 10 ng/mL rhIL21 for 2 hours . Data shown are for the 6 genes (of 19 tested) with the most consistent response among the 9 individual donors, and are identified as 6 preferred biomarkers of rhIL21 activity in whole human blood. These 6 genes were also identified as the best indicators of IL21 response in a series of pilot studies conducted with blood from a different group of donors (data not shown).

    Journal: Journal of Translational Medicine

    Article Title: Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

    doi: 10.1186/1479-5876-8-51

    Figure Lengend Snippet: Whole human blood response to ex vivo stimulation with 10 ng/mL rhIL21 for 2 hours . Data shown are for the 6 genes (of 19 tested) with the most consistent response among the 9 individual donors, and are identified as 6 preferred biomarkers of rhIL21 activity in whole human blood. These 6 genes were also identified as the best indicators of IL21 response in a series of pilot studies conducted with blood from a different group of donors (data not shown).

    Article Snippet: All treatments were run in duplicate. rhIL21 (produced from Chinese hamster ovary cells at Wyeth, now Pfizer) was added in volumes ranging from 3 μL to 10 μL to achieve the indicated concentration.

    Techniques: Ex Vivo, Activity Assay

    Determination of optimal time point for rhIL21-response for the 6 genes identified as preferred biomarkers . This time point experiment was done using 5 of the donors shown in Figure 2. Earlier pilot studies had suggested that stimulation for 30 minutes was significantly sub-optimal. There was no significant difference in response between all doses tested (30, 10, and 3 ng/mL). The lowest dose of rhIL21 that elicits a response has therefore not been determined.

    Journal: Journal of Translational Medicine

    Article Title: Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

    doi: 10.1186/1479-5876-8-51

    Figure Lengend Snippet: Determination of optimal time point for rhIL21-response for the 6 genes identified as preferred biomarkers . This time point experiment was done using 5 of the donors shown in Figure 2. Earlier pilot studies had suggested that stimulation for 30 minutes was significantly sub-optimal. There was no significant difference in response between all doses tested (30, 10, and 3 ng/mL). The lowest dose of rhIL21 that elicits a response has therefore not been determined.

    Article Snippet: All treatments were run in duplicate. rhIL21 (produced from Chinese hamster ovary cells at Wyeth, now Pfizer) was added in volumes ranging from 3 μL to 10 μL to achieve the indicated concentration.

    Techniques:

    Average percent inhibition of the expression level of 6 IL21-responsive genes . Percent inhibition values were calculated based on RQ (relative quantification) values of untreated control and rhIL21-treated samples for each of the 4 donors, and subsequently the mean and standard deviation were determined for each gene shown. A: Percent inhibition in presence of Ab-01. B: Percent inhibition in presence of control IgG 1 TM. Data for the 0.1 μg/mL , 0.3 μg/mL and 1 μg/mL concentrations were generated using 4 donors. Data for the higher and the lower concentrations were generated using 2 donors.

    Journal: Journal of Translational Medicine

    Article Title: Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

    doi: 10.1186/1479-5876-8-51

    Figure Lengend Snippet: Average percent inhibition of the expression level of 6 IL21-responsive genes . Percent inhibition values were calculated based on RQ (relative quantification) values of untreated control and rhIL21-treated samples for each of the 4 donors, and subsequently the mean and standard deviation were determined for each gene shown. A: Percent inhibition in presence of Ab-01. B: Percent inhibition in presence of control IgG 1 TM. Data for the 0.1 μg/mL , 0.3 μg/mL and 1 μg/mL concentrations were generated using 4 donors. Data for the higher and the lower concentrations were generated using 2 donors.

    Article Snippet: All treatments were run in duplicate. rhIL21 (produced from Chinese hamster ovary cells at Wyeth, now Pfizer) was added in volumes ranging from 3 μL to 10 μL to achieve the indicated concentration.

    Techniques: Inhibition, Expressing, Quantitative Proteomics, Control, Standard Deviation, Generated

    rhIL21-responsive genes in whole cynomolgus monkey blood

    Journal: Journal of Translational Medicine

    Article Title: Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

    doi: 10.1186/1479-5876-8-51

    Figure Lengend Snippet: rhIL21-responsive genes in whole cynomolgus monkey blood

    Article Snippet: All treatments were run in duplicate. rhIL21 (produced from Chinese hamster ovary cells at Wyeth, now Pfizer) was added in volumes ranging from 3 μL to 10 μL to achieve the indicated concentration.

    Techniques:

    Inhibition by Ab-01 of ex vivo rhIL21-dependent IL2RA expression

    Journal: Journal of Translational Medicine

    Article Title: Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

    doi: 10.1186/1479-5876-8-51

    Figure Lengend Snippet: Inhibition by Ab-01 of ex vivo rhIL21-dependent IL2RA expression

    Article Snippet: All treatments were run in duplicate. rhIL21 (produced from Chinese hamster ovary cells at Wyeth, now Pfizer) was added in volumes ranging from 3 μL to 10 μL to achieve the indicated concentration.

    Techniques: Inhibition, Ex Vivo, Control

    rhIL21 induced IL2RA expression in whole blood from cynomolgus monkeys dosed with Ab-01

    Journal: Journal of Translational Medicine

    Article Title: Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

    doi: 10.1186/1479-5876-8-51

    Figure Lengend Snippet: rhIL21 induced IL2RA expression in whole blood from cynomolgus monkeys dosed with Ab-01

    Article Snippet: All treatments were run in duplicate. rhIL21 (produced from Chinese hamster ovary cells at Wyeth, now Pfizer) was added in volumes ranging from 3 μL to 10 μL to achieve the indicated concentration.

    Techniques: Expressing, Control